Camping Lamp
Camping Lamp
Biochemistry problem!! Please Help?
Hi, the problem is a follows: The absorbance of a sample (enzyme and AMP) is 0.46 at 280nm and 0.58 at 260 nm. Calculate the concentration of enzyme and AMP given that.
For the enzyme the (molecular coefficient) is 2.96x10*4 at 280nm and 1.52x10*4 at 260nm.
The AMP has a molecular coefficient of 2.4x10*3 at 280nm and 1.5x10*4 at 260nm.
All these concentrations are in M cm. The path length is 1cm.
Now I know I should use the following equation.
A280nm = Eenz x Cenz x Lenz + Eamp x Camp x Lamp and the same for the wavelength at 260nm.
I know the answer for the enzyme is 1.36x10*-5M and AMP is 2.50x10*-5M. But my problem is I dot know how to manipulate the about equation to get the right answer.
Thanks a million for all your help!
In the equation given for A280, plug in the values, and you'll have 2 unknowns. One for Cenz and one for Camp. You can do the same for the equation for A260 and again plug in the values. You now have 2 equations with 2 unknowns, so you can solve one in terms of the other. That should be sufficient to solve for both unknowns, i.e. Cenz and Camp.
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