Camping Lamp
Camping Lamp
Biochemistry problem!! Please Help?
Hi, the problem is a follows: The absorbance of a sample (enzyme and AMP) is 0.46 at 280nm and 0.58 at 260 nm. Calculate the concentration of enzyme and AMP given that.
For the enzyme the (molecular coefficient) is 2.96x10*4 at 280nm and 1.52x10*4 at 260nm.
The AMP has a molecular coefficient of 2.4x10*3 at 280nm and 1.5x10*4 at 260nm.
All these concentrations are in M cm. The path length is 1cm.
Now I know I should use the following equation.
A280nm = Eenz x Cenz x Lenz + Eamp x Camp x Lamp and the same for the wavelength at 260nm.
I know the answer for the enzyme is 1.36x10*-5M and AMP is 2.50x10*-5M. But my problem is I dot know how to manipulate the about equation to get the right answer.
Thanks a million for all your help!
In the equation given for A280, plug in the values, and you'll have 2 unknowns. One for Cenz and one for Camp. You can do the same for the equation for A260 and again plug in the values. You now have 2 equations with 2 unknowns, so you can solve one in terms of the other. That should be sufficient to solve for both unknowns, i.e. Cenz and Camp.
 |
 10pcs 5W Power LED Miner Light Headlight Mining Lamp F Hunting Camping Fishing  US $390.00
|
 9W Cree LED Head Lamp Torch Light Headlamp Camping D9 US $329.99
|
 Authentic Models Hanging Camp Oil Lamp Lantern Brass Copper Anchor Light New US $209.99
|
 VAPALUX 300 LAMP TILLEY LAMP BIALADDIN LAMP PARAFFIN LAMP CAMPING LAMP US $195.22
|
 New Portable 2 Mode 12 LED Bivouac Camping Lantern Light Lamp Tent Fishing Torch US $160.00
|
 New Super Bright 7800mAh HID Lamp 8500LM SOS Xenon Flashlight Torch 85W Camping US $158.99
|
 24 LED Camping Light UFO Garden Parasol Lamp Umbrella Outdoor Patio Tent US $158.05
|
 Snowpeak GL 100A camping gas latern light lamp ourdoor fishing torch corefishing US $149.99
|
 Snowpeak GS 100A camping gas latern light lamp ourdoor fishing torch corefishing US $135.00
|
 LED Flashlight Waterproof Camping Torch Handheld Lamp US $129.00
|
 Snowpeak GL 150A camping gas latern light lamp ourdoor fishing torch corefishing US $125.00
|
 Super Bright HID Lamp 85W 8500LM SOS Xenon Flashlight Torch 7800mAh Camping Spot US $120.99
|
 10 x New 24 LED UFO Garden Patio Table Parasol Umbrella Pole Light Camping Lamp US $111.99
|
 Protable 12 LED Bivouac Camping Hiking Lantern Light Lamp US $100.99
|
 Vintage Coleman 220E Green Lantern Camping Gasoline White Gas Lamp 10 60 1960 US $100.00
|
 Vintage Coleman 242C Green Lantern Camping Gasoline White Gas Lamp 7 7 1940s US $100.00
|
 Vintage 1940s Coleman 242C Green Lantern Camping Gasoline Gas Lamp 7 7 US $100.00
|
 20 Brand New 9 LED Head Lamps CampingTrail Riding ETC US $94.99
|
 Adjustable Focus Zoom Cree AA LED Bright Lamp 5W Torch Flashlight Camping 9 New US $92.99
|
 3W LED Torch Lamp Flashlight Light for Hiking Camping US $91.93
|
 Camping Tree Tabletop Decoration Nightlight Woodsy Lamp Christmas Bear Camper US $89.95
|
 5 SETS Stowaway Lamps 10 1 Tool Camping Essential US $88.22
|
 10 Solar Camping Lantern or Lamp Charges Cell Phones US $84.99
|
 12 LED LAMP FLASHLIGHT WHOLESALE LOT WORK CAMPING HIKE US $79.99
|
 Vintage Coleman 228F 220F Green Lantern Camp Gasoline White Gas Lamp 6 66 1966 US $70.00
|
 RECHARGEABLE LANTERN LAMP FOR CAMPING OUTDOOR LIGHTS NIGHT RETRO STYLE RED NEW US $67.99
|
Mini Camping Lamp with Digital FM Radio
